Case Study
A 62-year-old male presented to his physician with
petechiae, epistaxis and exhaustion. STAT blood work revealed a marked thrombocytopenia.
Laboratory Findings: Peripheral Blood
|
16.4 X 109/L |
[4.0-10.0 X 109/L] |
|
77 g/L |
[135-170 g/L] |
|
<10 X 109/L |
[150-400 X 109/L] |
Automated Differential: Peripheral Blood
|
14.0X 109/L |
[2.0-7.5 X 109/L] |
|
1.5 X 109/L |
[1.5-4.0 /109/L] |
|
0.6 X 109/L |
[0.2-0.8 X 109/L] |
|
*Manual differential shows predominantly “monocytes” and 13% blast cells. |
||
The bone marrow was sent for flow cytometric analysis. Refer to
photomicrographs and discussion
for bone marrow morphology. Listmode files and the corresponding antibody combinations are shown below.
| Listmode ID | 4-Colour Antibody Combination |
|
Z0015159
|
*CONTROL Cells Only
FITC/PE/ECD/CD45PC5 |
|
Z0015160
|
CD8FITC/CD4PE/CD3ECD/CD45PC5
|
|
Z0015161
|
CD14FITC/CD16PE/CD45 ECD/CD64PC5
|
|
Z0015162
|
HLADRFITC/CD13PE/CD45ECD/CD15PC5
|
|
Z0015163
|
CD19FITC/CD10PE/CD45ECD/CD20PC5
|
| 3-Colour Antibody Combination | |
|
Z0015164
|
BlankFITC/BlankPE/BlankECD/CD45PC5
|
|
Z0015165
|
CD8FITC/CD4PE/CD3ECD/CD45PC5
|
|
Z0015167
|
Intracellular control IgGFITC/PE/ECD/CD45PC5
|
|
Z0015168
|
Intracellular |