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Case Study
A
28-year-old man presented to his physician’s office with an enlarged lymph
node in the right side of his neck. He was sent for a lymph node aspiration, the results
suggested that it was reactive in nature. Upon follow-up six months later, he
presented with a jugulodigastric node of approximately 1 cm in size. A fine
needle aspirate was sent for flow cytometric analysis. At this time his CBC was
within normal limits.
Laboratory Findings: Peripheral Blood
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5.1 X 109/L
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[4.0-10.0 X 109/L]
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152 g/L
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[135-170 g/L]
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93.6 fL
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[79.0-97.0 fL]
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196 X 109/L
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[150-400 X 109/L]
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Differential: Peripheral Blood
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2.8X 109/L
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[2.0-7.5 X 109/L]
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1.9 X 109/L
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[1.5-4.0 /109/L]
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0.4X 109/L
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[0.2-0.8 X 109/L]
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* Differential shows marked left shift
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This case includes 2 lymph node aspirate photomicrographs. The lymph node aspirate was sent for
flow cytometric analysis. Listmode files and the corresponding antibody
combinations are shown below.
| Listmode ID |
4-Colour
Antibody Combination |
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Z0009969
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CONTROL Cells Only FITC/PE/ECD/CD45PC5
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Z0009970
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CD8FITC/CD4PE/CD3ECD/CD45PC5
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Z0009971
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CD5FITC/CD10PE/CD19ECD/CD45PC5
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Z000972
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FMCFITC/CD23PE/CD19ECD/CD45PC5
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Z0009973
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CD20FITC/CD2PE/ECD/CD45PC5
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3-Colour
Antibody Combination
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Z0009980
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FABFITC/CD19PE/CD45PC5
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Z0009981
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KAPPAFITC/CD19PE/CD45PC5
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Z0009982
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LAMBDAFITC/CD19PE/CD45PC5
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