Case Study

 A 35-year-old male had a two-week history of easy bruising, hematuria, gum bleeding and was generally unwell. He presented to a walk-in clinic for treatment. STAT blood work revealed a marked thrombocytopenia, with immature myeloid cells present on the blood smear.

Laboratory Findings: Peripheral Blood
· WBC 16.1 X 109/L [4.0-10.0 X 109/L]
· Hb 95 g/L [13.5-17.0 g/L]
· Plt 14 X 109/L [150-400 X 109/L]

Differential: Peripheral Blood

· Neutrophil 14.0 X 109/L* [2.0-7.5 X 109/L]
· Lymphocyte 1.5 X 109/L [1.5-4.0 /109/L]
· Monocyte 0.6 X 109/L [0.2-0.8 X 109/L]
* Differential shows marked left shift


The bone marrow was sent for flow cytometric analysis. Listmode files and the corresponding antibody combinations are shown below:

 

Listmode ID 3-Colour Antibody Combination
Z0156999
CONTROL Cells Only FITC/PE/CD45PC5
Z0157000
CD36FITC/CD14(M02PE)/CD45PC5
Z0157001
CD8FITC/CD4PE/CD45PC5
Z0157002
CD10FITC/CD19PE/CD45PC5
Z0157003
CD20FITC/2PE/CD45PC5
Z0157004
CD16FITC/13PE/CD45PC5
Z0157005
CD7FITC/HLA-DRPE/CD45PC5
Z0157006
CD34FITC/33PE/CD45PC5
Z0157007
CD15FITC/13PE/CD45PC5
Z0157008
CD64FITC/CD14(MY4PE)/CD45PC5
Z0157027
Intracellular control IgGFITC/PE/ECD/CD45PC5
Z0157028
Intracellular MPOFITC/CD79aPE/CD3ECD/CD45PC5
 
Intro
Photomicrograph
Scatterplots
Summary


©2002 QMP–LS (Department of the OMA). All rights reserved